Plant annexins form calcium-independent oligomers in solution
نویسندگان
چکیده
منابع مشابه
Plant annexins form calcium-independent oligomers in solution.
The oligomeric state in solution of four plant annexins, namely Anx23(Ca38), Anx24(Ca32), Anx(Gh1), and Anx(Gh2), was characterized by sedimentation equilibrium analysis and gel filtration. All proteins were expressed and purified as amino-terminal His(n) fusions. Sequencing of the Anx(Gh1) construct revealed distinct differences with the published sequence. Sedimentation equilibrium analysis o...
متن کاملAnnexins as Overlooked Regulators of Membrane Trafficking in Plant Cells
Annexins are an evolutionary conserved superfamily of proteins able to bind membrane phospholipids in a calcium-dependent manner. Their physiological roles are still being intensively examined and it seems that, despite their general structural similarity, individual proteins are specialized toward specific functions. However, due to their general ability to coordinate membranes in a calcium-se...
متن کاملAnnexins - calcium- and membrane-binding proteins in the plant kingdom: potential role in nodulation and mycorrhization in Medicago truncatula.
Annexins belong to a family of multi-functional membrane- and Ca(2+)-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca(2+)-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant counterparts were discovered only in 1989, in tomato, and have not been thoro...
متن کاملAnnexins: components of the calcium and reactive oxygen signaling network.
Despite the importance of reactive oxygen species (ROS) in plant immunity, stress signaling, and development (Mori and Schroeder, 2004; Gadjev et al., 2006), the molecular identities of Ca-permeable channels responding to ROS have not been established. Here, we propose annexins as candidate channel proteins. Elevation of cytoplasmic free calcium ([Ca2+]cyt) as a regulatory step in plant immunit...
متن کاملPurification and partial sequence analysis of plant annexins.
A fractionation procedure for annexins involving Ca2(+)-dependent binding to exogenous phospholipid was applied to tomato suspension culture cells. Two polypeptides (34 kDa and 35.5 kDa) were purified and separated from each other and from contaminant pectic polysaccharide by ion-exchange chromatography. After proteolytic digestion of SDS/PAGE-purified products, N-terminal sequencing of the pep...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Science
سال: 2002
ISSN: 0961-8368
DOI: 10.1110/ps.4770102